Dideoxy DNA sequencing reaction [videorecording] / Taped Technologies production
Publication details: Logan, Utah : Taped Technologies, 1994Description: 1 videocassette (23 min) : sd., col. ; 1/2 inSubject(s): NLM classification:- QU58
| Item type | Current library | Home library | Call number | Materials specified | Copy number | Status | Date due | Barcode | |
|---|---|---|---|---|---|---|---|---|---|
| MEDIA | PERPUSTAKAAN DR ABDUL LATIFF | PERPUSTAKAAN DR ABDUL LATIFF MEDIA-P. DR ABDUL LATIFF | QU58.D555 1994 video (Browse shelf(Opens below)) | 1 | Available | 00001103336 |
The dideoxy DNA sequencing reaction is considered to be the least labor-intensive and most practical method for routine DNA sequencing. The components of 4 sequencing reactions consisted of a DNA template annealed to a prime, Klenow DNA polymerase, deoxynucleotides, and 35S-dATP. Each of the different reactions contained one of four chain terminators - dideoxy GTP, dideoxy ATP, dideoxy TTP, or dideoxy CTP. Consequently, the lengths of the radiolabeled reaction products corresponded to every nucleotide in the sequence. The reactions were stored indefinitely in a formamide buffer until ready for sequencing.
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