MARC details
| 000 -LEADER |
|---|
| fixed length control field |
01502ngm a2200265 a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20250913120627.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
981208q1994 xxu023 0v eng b |
| 039 #9 - LEVEL OF BIBLIOGRAPHIC CONTROL AND CODING DETAIL [OBSOLETE] |
|---|
| Level of rules in bibliographic description |
200810291755 |
| Level of effort used to assign nonsubject heading access points |
midah |
| Level of effort used to assign subject headings |
200211201122 |
| Level of effort used to assign classification |
baizura |
| y |
08-18-1999 |
| z |
load |
| 060 ## - NATIONAL LIBRARY OF MEDICINE CALL NUMBER |
|---|
| Classification number |
QU58 |
| Item number |
.D555 1994 video 9 |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
QU58.D555 1994 video |
| 245 00 - TITLE STATEMENT |
|---|
| Title |
Dideoxy DNA sequencing reaction |
| Medium |
[videorecording] / |
| Statement of responsibility, etc. |
Taped Technologies production |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Logan, Utah : |
| Name of publisher, distributor, etc. |
Taped Technologies, |
| Date of publication, distribution, etc. |
1994 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
1 videocassette (23 min) : |
| Other physical details |
sd., col. ; |
| Dimensions |
1/2 in |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
The dideoxy DNA sequencing reaction is considered to be the least labor-intensive and most practical method for routine DNA sequencing. The components of 4 sequencing reactions consisted of a DNA template annealed to a prime, Klenow DNA polymerase, deoxynucleotides, and 35S-dATP. Each of the different reactions contained one of four chain terminators - dideoxy GTP, dideoxy ATP, dideoxy TTP, or dideoxy CTP. Consequently, the lengths of the radiolabeled reaction products corresponded to every nucleotide in the sequence. The reactions were stored indefinitely in a formamide buffer until ready for sequencing. |
| 650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Base Sequence |
| 650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Dideoxynucleosides |
| 650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
DNA |
| General subdivision |
Analysis |
| 907 ## - LOCAL DATA ELEMENT G, LDG (RLIN) |
|---|
| a |
.b10554725 |
| b |
2019-11-12 |
| c |
2019-11-12 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
MEDIA |
| Suppress in OPAC |
No |
| Call number prefix |
QU58.D555 1994 video |
| 914 ## - VTLS Number |
|---|
| VTLS Number |
vtls000057720 |
| 990 ## - EQUIVALENCES OR CROSS-REFERENCES [LOCAL, CANADA] |
|---|
| Link information for 9XX fields |
nbz |
| 991 ## - LOCAL NOTE (NAMA FAKULTI/INSTITUT/PUSAT) |
|---|
| a |
Perpustakaan Perubatan |
| 998 ## - LOCAL CONTROL INFORMATION (RLIN) |
|---|
| Library |
PERPUSTAKAAN DR ABDUL LATIFF |
| Operator's initials, OID (RLIN) |
1999-05-08 |
| Cataloger's initials, CIN (RLIN) |
m |
| Material Type (Sierra) |
Video/ DVD |
| Language |
English |
| Country |
|
| -- |
0 |
| -- |
.b10554725 |
