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| 100 | 1 | _aFreshney, R. Ian. | |
| 245 | 1 | 0 |
_aCulture of animal cells : _ba manual of basic technique and specialized applications / _cR. Ian Freshney. |
| 250 | _a6th ed. | ||
| 260 |
_aHoboken, N.J. : _bWiley-Blackwell, _c©2010. |
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| 300 |
_a1 online resource (xxxi, 732 pages, [28] pages of plates) : _billustrations (some color) |
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| 336 |
_atext _2rdacontent |
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| 337 |
_acomputer _2rdamedia |
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| 338 |
_aonline resource _2rdacarrier |
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| 504 | _aIncludes bibliographical references (pages 663-715) and index. | ||
| 505 | 0 | _aFrontmatter -- Color Plates -- Introduction -- Biology of Cultured Cells -- Laboratory Design, Layout, and Equipment -- Equipment and Materials -- Aseptic Technique -- Safety, Bioethics, and Validation -- Culture Vessels and Substrates -- Defined Media and Supplements -- Serum-Free Media -- Preparation and Sterilization -- Primary Culture -- Subculture and Cell Lines -- Cloning and Selection -- Cell Separation -- Characterization -- Differentiation -- Transformation and Immortalization -- Contamination -- Cryopreservation -- Quantitation -- Cytotoxicity -- Specialized Cells -- Stem Cells, Germ Cells, and Amniocytes -- Culture of Tumor Cells -- Three-Dimensional Culture -- Scale-Up and Automation -- Specialized Techniques -- Training Programs -- Problem Solving -- In Conclusion -- Appendix I: Calculations and Preparation of Reagents -- Appendix II: Sources of Equipment and Materials -- Appendix III: Suppliers and Other Resources -- Appendix IV: Glossary -- Appendix V: Cross-contaminated or Misidentified Cell Lines -- Appendix VI: General Textbooks and Relevant Journals -- References -- Index. | |
| 520 | _aThis is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture. Since publication of the previous edition of this benchmark text, numerous types of equipment and materials have changed and new specialized techniques have been developed, particularly in the area of stem cell research and tissue engineering. These and other developments have been incorporated into this rigorously revised and expanded sixth edition of Culture of Animal Cells, the leading text in the basic methodology of cell culture. | ||
| 588 | 0 | _aPrint version record. | |
| 650 | 0 |
_aTissue culture _vLaboratory manuals. |
|
| 650 | 0 |
_aCell culture _vLaboratory manuals. |
|
| 650 | 1 | 2 | _aCells, Cultured. |
| 650 | 2 | 2 |
_aCulture Techniques _xmethods. |
| 655 | 4 | _aElectronic books. | |
| 655 | 7 |
_aLaboratory manuals. _2fast _0(OCoLC)fst01920776 |
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| 710 | 2 | _aWiley InterScience (Online service) | |
| 773 | 0 | _tWiley e-books. | |
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_iPrint version: _aFreshney, R. Ian. _tCulture of animal cells. _b6th ed. _dHoboken, N.J. : Wiley-Blackwell, ©2010 _z9780470528129 _w(OCoLC)708738135 |
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_aWiley Online Library. _pBooks. |
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_uhttps://eresourcesptsl.ukm.remotexs.co/user/login?url=http://onlinelibrary.wiley.com/book/10.1002/9780470649367 _zWiley Online Library |
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