| 000 | 01502ngm a2200265 a 4500 | ||
|---|---|---|---|
| 005 | 20250913120627.0 | ||
| 008 | 981208q1994 xxu023 0v eng b | ||
| 039 | 9 |
_a200810291755 _bmidah _c200211201122 _dbaizura _y08-18-1999 _zload |
|
| 060 |
_aQU58 _b.D555 1994 video 9 |
||
| 090 | _aQU58.D555 1994 video | ||
| 245 | 0 | 0 |
_aDideoxy DNA sequencing reaction _h[videorecording] / _cTaped Technologies production |
| 260 |
_aLogan, Utah : _bTaped Technologies, _c1994 |
||
| 300 |
_a1 videocassette (23 min) : _bsd., col. ; _c1/2 in |
||
| 520 | _aThe dideoxy DNA sequencing reaction is considered to be the least labor-intensive and most practical method for routine DNA sequencing. The components of 4 sequencing reactions consisted of a DNA template annealed to a prime, Klenow DNA polymerase, deoxynucleotides, and 35S-dATP. Each of the different reactions contained one of four chain terminators - dideoxy GTP, dideoxy ATP, dideoxy TTP, or dideoxy CTP. Consequently, the lengths of the radiolabeled reaction products corresponded to every nucleotide in the sequence. The reactions were stored indefinitely in a formamide buffer until ready for sequencing. | ||
| 650 | 2 | _aBase Sequence | |
| 650 | 2 | _aDideoxynucleosides | |
| 650 | 2 |
_aDNA _xAnalysis |
|
| 907 |
_a.b10554725 _b2019-11-12 _c2019-11-12 |
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| 942 |
_c5 _n0 _kQU58.D555 1994 video |
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| 914 | _avtls000057720 | ||
| 990 | _anbz | ||
| 991 | _aPerpustakaan Perubatan | ||
| 998 |
_ad _b1999-05-08 _cm _dg _feng _gxxu _y0 _z.b10554725 |
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| 999 |
_c57394 _d57394 |
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