Includes bibliographical references (pages 663-715) and index.
Frontmatter -- Color Plates -- Introduction -- Biology of Cultured Cells -- Laboratory Design, Layout, and Equipment -- Equipment and Materials -- Aseptic Technique -- Safety, Bioethics, and Validation -- Culture Vessels and Substrates -- Defined Media and Supplements -- Serum-Free Media -- Preparation and Sterilization -- Primary Culture -- Subculture and Cell Lines -- Cloning and Selection -- Cell Separation -- Characterization -- Differentiation -- Transformation and Immortalization -- Contamination -- Cryopreservation -- Quantitation -- Cytotoxicity -- Specialized Cells -- Stem Cells, Germ Cells, and Amniocytes -- Culture of Tumor Cells -- Three-Dimensional Culture -- Scale-Up and Automation -- Specialized Techniques -- Training Programs -- Problem Solving -- In Conclusion -- Appendix I: Calculations and Preparation of Reagents -- Appendix II: Sources of Equipment and Materials -- Appendix III: Suppliers and Other Resources -- Appendix IV: Glossary -- Appendix V: Cross-contaminated or Misidentified Cell Lines -- Appendix VI: General Textbooks and Relevant Journals -- References -- Index.
This is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture. Since publication of the previous edition of this benchmark text, numerous types of equipment and materials have changed and new specialized techniques have been developed, particularly in the area of stem cell research and tissue engineering. These and other developments have been incorporated into this rigorously revised and expanded sixth edition of Culture of Animal Cells, the leading text in the basic methodology of cell culture.