TY  - ADVS
TI  - Dideoxy DNA sequencing reaction
PY  - 1994///
CY  - Logan, Utah
PB  - Taped Technologies
KW  - Base Sequence
KW  - Dideoxynucleosides
KW  - DNA
KW  - Analysis
N2  - The dideoxy DNA sequencing reaction is considered to be the least labor-intensive and most practical method for routine DNA sequencing. The components of 4 sequencing reactions consisted of a DNA template annealed to a prime, Klenow DNA polymerase, deoxynucleotides, and 35S-dATP. Each of the different reactions contained one of four chain terminators - dideoxy GTP, dideoxy ATP, dideoxy TTP, or dideoxy CTP. Consequently, the lengths of the radiolabeled reaction products corresponded to every nucleotide in the sequence. The reactions were stored indefinitely in a formamide buffer until ready for sequencing
ER  -