Dideoxy DNA sequencing reaction  [videorecording] / 
Taped Technologies production 
 - Logan, Utah :  Taped Technologies,  1994 
 - 1 videocassette (23 min) :  sd., col. ;  1/2 in 
<br/><br/> The dideoxy DNA sequencing reaction is considered to be the least labor-intensive and most practical method for routine DNA sequencing. The components of 4 sequencing reactions consisted of a DNA template annealed to a prime, Klenow DNA polymerase, deoxynucleotides, and 35S-dATP. Each of the different reactions contained one of four chain terminators - dideoxy GTP, dideoxy ATP, dideoxy TTP, or dideoxy CTP. Consequently, the lengths of the radiolabeled reaction products corresponded to every nucleotide in the sequence. The reactions were stored indefinitely in a formamide buffer until ready for sequencing. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Base Sequence<br/>Dideoxynucleosides<br/>DNA--Analysis
<br/><br/><label>National Library of Medicine Call No.: </label>QU58  / .D555 1994 video 9